In the late fall of 1597, Guale Indians murdered five Franciscan friars stationed in their territory and razed their missions to the ground. The 1597 Guale Uprising, or Juanillo's Revolt as it is often labeled, brought the missionization of Guale to an abrupt end and threatened Florida's new governor with the most significant crisis of his term. This lecture by Dr. J. Michael Francis explores the 1597 uprising and its aftermath, and aims to shed light on the complex nature of Spanish-Indian relations in early colonial Florida.